Changes

�*Cells in 100 mm culture dishes�*Labelling Media (Inositol Free DMEM with PSG, 10% dialysed serum and 100 uCi inositol per plate

�*Perchloric acid (640 uL into 10 mL water) keep cold�*100 mM EDTA (2 mL of 0.5M stock in 10 mL) keep cold�*Water�*Deacylation Mix (3.5 mL Methylamine, 6 mL Methanol, 1.5 mL Butanol, 2.1 mL Water)�*Lipid Extraction Mix (10 mL Butanol, 2 mL Ethyl Ether and 0.5 mL Ethyl Formate)