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Measuring Fasting Insulin

269 bytes removed, 18 January
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==Materials==
*Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)
*Heparinzed capilaries (Fisher cat# 22-362574)
==Protocol==
#Collect about 2/3 of a capillary of blood, typically via a retro-orbital bleed.#Transfer blood to labelled eppendorf tubes.#Let blood clot for 30 min on ice.#Centrifuge 20 min at 2000g serum (about 5000 RPM in eppendorf 5415C centrifugesee [[Collecting_and_Storing_Mouse_Serum]]).#Gentry remove liquid layer and store at -80