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Created page with "== Cholesterol Assay == === Materials === * Reagent * Cholesterol Standard === Protocol === * Make 10X stock dilution: Add 50ɥL Cholesterol Standard 450ɥL water to a 1.5mL..."
== Cholesterol Assay ==
=== Materials ===
* Reagent
* Cholesterol Standard
=== Protocol ===
* Make 10X stock dilution: Add 50ɥL Cholesterol Standard 450ɥL water to a 1.5mL tube
* Pipette 100ɥL of Reagent to 22 wells
* Add 2-10ɥL of 10X stock dilution to wells in columns 2-6 in increments of 2ɥL. Each column will have 2 replicates per volume (1 replicate per row).
* Repeat in columns 7-11 with 2-10ɥL of Cholesterol Standard (1X dilution)
* Allow reaction to proceed for 15 minutes
* Read absorbance
=== Materials ===
* Reagent
* Cholesterol Standard
=== Protocol ===
* Make 10X stock dilution: Add 50ɥL Cholesterol Standard 450ɥL water to a 1.5mL tube
* Pipette 100ɥL of Reagent to 22 wells
* Add 2-10ɥL of 10X stock dilution to wells in columns 2-6 in increments of 2ɥL. Each column will have 2 replicates per volume (1 replicate per row).
* Repeat in columns 7-11 with 2-10ɥL of Cholesterol Standard (1X dilution)
* Allow reaction to proceed for 15 minutes
* Read absorbance