Changes

Finding and Ordering BAC Clones

347 bytes added, 15:26, 27 July 2009
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#Zoom out as necessary to find ends of BAC clones
#Print out and save BAC clone choices
 
==Find Clone with BLAST==
#Go to blast website (http://www.ncbi.nlm.nih.gov/blast)
#Select Mouse (or whichever species) under BLAST assembled genomes
#Select clone end sequences under database
#Select appropriate program (BLASTN if using a nucleotide query, TBLASTN if using a protein query)
#Enter sequence or accession number in the search box
==Ordering BAC Clones==
#Go to BACPAC website [(http://bacpac.chori.org/order_clones.php])
#Enter clone name and submit to verify
#Can order clones through LSI purchasing (vendor is listed as BACPAC-chor)