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→RT-PCR Reaction
===RT-PCR Reaction===
#Book PCR Machine for 2h
#Thaw, mix and quickly spin RNA, dNTP mix, random hexamers, 10X RT buffer, 25 mM MgCl2 water. All reagents are in the small red invitrogen box#Combine the followingin a PCR tube:
##8 uL RNA
##1 uL dNTP mix