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!| PCR Step || Temperature || Time || Cycles || Notes
|-
|1. Initiation/Melting || 94 || 5:00 || 1 ||
|-
|2. Denaturation || 94 || 0:45 || 40 ||
|-
|3. Annealing || 66 || 0:45 || 40 ||
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|4. Elongation || 72 || 0:45 || 40 || steps 2-4 to be run in sequence
|-
|5. Amplification || 72 || 7:00 || 1 ||
|-
|6. Finish || 4 || infinite || n/a ||
|}