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==PRIMERS==
*Fwd: CAC TCG GAG CTT CCC TGA CCC AG
*Rev: GAC CAT GGT TTC CGA CCC ATT
*Neo Rev: TTC CAA GCC TCT GAG CCC AGA
Make primers at 1uM (Fwd + Rev and Fwd + Neo Rev). PCR primers separately.
==PCR PROTOCOL==
{|border="1"
!| PCR Step || Temperature || Time || Cycles || Notes
|-
1. Initiation/Melting || 94 || 5:00 || 1 ||
|-
2. Denaturation || 94 || 0:45 || 40 ||
|-
3. Annealing || 66 || 0:45 || 40 ||
|-
4. Elongation || 72 || 0:45 || 40 || steps 2-4 to be run in sequence
|-
5. Amplification || 72 || 7:00 || 1 ||
|-
6. Finish || 4 || infinite || n/a ||
|}
[[ Category:Genotyping ]]
[[ Category:Mouse Work]]
[[ Category:PCR]]
[[ Category:DNA]]
[[ Category:Molecular Biology]]
*Fwd: CAC TCG GAG CTT CCC TGA CCC AG
*Rev: GAC CAT GGT TTC CGA CCC ATT
*Neo Rev: TTC CAA GCC TCT GAG CCC AGA
Make primers at 1uM (Fwd + Rev and Fwd + Neo Rev). PCR primers separately.
==PCR PROTOCOL==
{|border="1"
!| PCR Step || Temperature || Time || Cycles || Notes
|-
1. Initiation/Melting || 94 || 5:00 || 1 ||
|-
2. Denaturation || 94 || 0:45 || 40 ||
|-
3. Annealing || 66 || 0:45 || 40 ||
|-
4. Elongation || 72 || 0:45 || 40 || steps 2-4 to be run in sequence
|-
5. Amplification || 72 || 7:00 || 1 ||
|-
6. Finish || 4 || infinite || n/a ||
|}
[[ Category:Genotyping ]]
[[ Category:Mouse Work]]
[[ Category:PCR]]
[[ Category:DNA]]
[[ Category:Molecular Biology]]