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#Vortex vigorously then sit at room temperature for 5 minutes
#Centrifuge for 10 minutes @ 13000G
#Transfer 400 200 ul of the bottom layer into a new tube#Centrifuge again for 7-10 minutes @ 13000G#Transfer 150 ul of the bottom layer into the new tube (if there is enough of the bottom layer, transfer a total of 200 ul)
#Let evaporate overnight at room temperature
#Add'''(50ul)''' of '''Butanol Mixture''' and vortex. See Suggested Volumes for your specific tissue.