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QPCR Analysis on ThermoCloud

14 bytes added, 16:49, 4 December 2018
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**Verify that each of your replicates amplified for each target.
**If there is a target that yields 2+ peaks over all samples, mark that target as unusable in the '''Primer Database'''.
**Exclude samples that did not pass the above criteria by clicking the well (not the plate schematic ) and select '''Omit''' under the '''Action''' dropdown box above the plate schematic.
*Check the technical replicates by choosing the '''Table View''' and arranging them according to target. Verify that the '''CT''' values have <1.5 difference. Omit outliers.
*Click '''Next''' and '''Export''' your results to the appropriate project folder.
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