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Created page with "== Tris Buffer .2M pH 7.4 == #Warm Tris buffer to 37°C in water bath. #Use hot plate to keep the buffer at 37°C, pH the buffer with HCl. (pH changes with temperature) ==Met..."
== Tris Buffer .2M pH 7.4 ==
#Warm Tris buffer to 37°C in water bath.
#Use hot plate to keep the buffer at 37°C, pH the buffer with HCl. (pH changes with temperature)
==Methods==
#20mg NBT aka Nitrotetrazolium Blue Chloride or Nitro Blue Tetrazolium
#80mg of NADH aka β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate
#100mL of Tris Buffer
#100mL fits in a staining dumpster
#Incubate for 30 minutes at 37°C
== Dehydrate and coverslip ==
#Rinse 3-4 times under running DI water
#Immerse in 50% ethanol for 2x 30 seconds
#Immerse in 70% ethanol for 2x 30 seconds
#Immerse in in 95% ethanol for 2x 30 seconds
#Immerse in in 100% ethanol for 2x 30 seconds
#Immerse in 1:1 xylene ethanol for 2 minutes for 2x 30 seconds
#Repeat twice more using fresh xylene. Check staining under the microscope
#Coverslip using Permount
#Warm Tris buffer to 37°C in water bath.
#Use hot plate to keep the buffer at 37°C, pH the buffer with HCl. (pH changes with temperature)
==Methods==
#20mg NBT aka Nitrotetrazolium Blue Chloride or Nitro Blue Tetrazolium
#80mg of NADH aka β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate
#100mL of Tris Buffer
#100mL fits in a staining dumpster
#Incubate for 30 minutes at 37°C
== Dehydrate and coverslip ==
#Rinse 3-4 times under running DI water
#Immerse in 50% ethanol for 2x 30 seconds
#Immerse in 70% ethanol for 2x 30 seconds
#Immerse in in 95% ethanol for 2x 30 seconds
#Immerse in in 100% ethanol for 2x 30 seconds
#Immerse in 1:1 xylene ethanol for 2 minutes for 2x 30 seconds
#Repeat twice more using fresh xylene. Check staining under the microscope
#Coverslip using Permount