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PAS Staining

697 bytes added, 20:30, 16 July 2018

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b) Pre-prepared Schiff’s reagent - Australian Biostain Pty Ltd,PO Box 1407,Traralgon, VIC.

==Sigma-Aldrich Protocol==

*Using PAS Staining System: 395B-1KT

#Deparaffinze and hydrate sections to deionized water.

#Immerse slides in '''Periodic Acid Solution''' for 5 minutes at room temperature (18-26C).

#Rinse slide in several changes of distilled water.

#Immerse slides in '''Schiff's Reagent''' for 15 minutes at room temperature (18-26C).

#Wash slides in running tap water for 5 minutes.

#Counterstain slides in '''Hematoxylin Solution''', Gill No. 3, for 90 seconds.

#Rinse slides in running tap water.

#Dehydrate, clear, and mount sections in toluene or xylene based mounting media.

https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/General_Information/2/395.pdf

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