915
edits
Changes
Updated with product details
==Materials==
*Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)
*Heparinzed capilaries(Fisher cat# 22-362574)
==Protocol==
#Collect about 2/3 of a capillary of blood, typically via a retro-orbital bleed.
#Transfer blood to labelled eppendorf tubes.
#Let blood clotfor 30 min on ice.#Centrifuge 20 min at 2000g (about 5000 RPM in eppendorf 5415C centrifuge).#Gentry remove liquid layer and store at -80