* 1.125g 0.45% Tween 20
1. Start by adding 150ml ddH2O to a beaker on a stir plate (with magnetic stir bar).
2# Start by adding 150ml ddH2O to a beaker on a stir plate (with magnetic stir bar). # Add reagents. Mix well. 3. # Add 37% HCl drop-wise to adjust pH to 8.3. 4. # Add ddH20 to 250ml.
==Protocol==