915
edits
Changes
no edit summary
#After 5 min add 50 uL cold 2-DG
#Wash cells 3x1mL with cold PBS
#Add 500 uL PBS per well#Scrape cells with a an upside down p200 tip
#Add 400 uL cells to 5 mL aqueous scintillation fluid, vortex and count on program 3
#Do a bradford assay on 20 uL of cells (use PBS as blank)