Changes

## Add 700 100 uL of Glucose Buffer Solution with Color Reagent buffer solution to a plastic cuvette. (each well, including wells for microplate add 100ul)standard curve and blank## Add 1-5 uL glucose standard (500mg/dL) for standard curve (for microplate add 1-5 ul of glucose standard 200mg/dL diluted 1:5)## Add 10 uL digested glycogen (for mice fasted more than 6 hours. samples, and use a 10X dilution for fed/short fast need lessrefed tissues (using either 5 or 2ul from the diluted sample)