52
edits
Changes
QPCR
,added how to make the 100 uM stock: "-the 100uM stock is prepared by adding 227 uL of distilled water to 22.7nmol of a gene as an example-"
*SYBR Green PCR Master Mix Applied Biosystems (ThermoFisher Catalog # 4367659; [https://www.thermofisher.com/order/catalog/product/4367659 Vendor Link])
*384 well qPCR plate (ThermoFisher Catalog # 4309849) and covers (Catalog # 4360954)
*Primers (Dilute to 0.4 uM mixture of fwd and rev. From 100 uM stocks - the 100uM stock is prepared by adding 227 uL of distilled water to 22.7nmol of a gene as an example- this is 4uL Forward Primer, 4 uL Reverse Primer and 992 uL Water). This primer mix can be stored at -20 in a Working Primers box. Design primers according to [[Primer Design for qPCR]]
===Plate Preparation===