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First Strand cDNA Synthesis (AB Kit)

9 bytes added, 15:33, 8 May 2017

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* Add 10 uL Master mix per well into tube or plate

* Add 0.1-10 ug RNA in a final volume of 10 uL to each well

* Run the PCR Program '''AB ReactionProtocol'''. Takes ~2h:

** 10 min at 25C

** 120 min at 37C

** 5 min at 85C

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