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Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)

339 bytes added, 13:17, 20 May 2009
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'''Materials'''
#0.1M NaBicarbonate (0.8401g in 100mL ddH<sub>2</sub>O) and 0.5MNaCl (2.922g in 100mL ddH<sub>2</sub>O)at pH 8.0.#50mM Sodium Acetate (0.41g in 100mL ddH<sub>2</sub>O)at pH 4.0#50mM Tris (0.61g in 100mL ddH<sub>2</sub>O) and .5M NaCl (2.922g in 100mL ddH<sub>2</sub>O) at pH 8.0#0.4M glycine (3.004g in 100mL ddH<sub>2</sub>O)at pH 1.8. '''Procedure'''Protein must be extensively dialysed in PPBS to remove any amine group from the sample. #
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