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→Experimental protocol
Note: Mice do not need to be in a fasted state prior to this test.
#Briefly anesthetize mice with isofluorane and collect blood via retro orbital bleed. Allow blood to clot over ice.
#Inject 10 mg/kg isoproterenol (prepared fresh in sterile PBS) via intraperitoneal injection. This will be 10mg in 10mL then inject 10uL/g mouse.
#Wait 15 minutes.
#Briefly anesthetize mice with isoflurane and collect blood via retro orbital bleed. Allow blood to clot over ice. ***Note: Take care when anesthetizing mice the second time. They will go under VERY quickly, so watch the mouse closely***