162
edits
Changes
→Incubate bead-antibody complex with fragmented, cross-linked chromatin
# Collect beads containing immuno-bound chromatin by placing the microfuge tube on a magnet rack.
# Remove and discard supernatant.
# Wash beads 5 times with 1 mL LiCl Wash Buffer, mixing 3 minutes for each wash on a rotator.
# Add 1 ml TE Buffer to beads. Mix 1 minute on rotator and then place tubes on magnet rack to collect beads and discard supernatant.
# Resuspend the bead pellet in 200 μl IP Elution Buffer (at room temperature). Vortex to mix.