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• Surface Plasmon Resonance - Protein Lipid Interactions
  #Inject protein samples adjusting contact time as necessary to reach saturation. Typically [[Category: Protein-Lipid Interactions]]
  5 KB (753 words) - 14:40, 14 April 2011
• Preparation of Protein Lysates from Mouse Tissues
  #Follow Protein Lysate instructions for Bradford Assay (see [[Bradford Assay]]) ...2X loading buffer with B-ME to each lyaste. This will generate a 2 mg/mL protein solution in SDS Loading Buffer
  1 KB (253 words) - 16:06, 25 April 2019
• Immobilizing a Protein on a CM5 Chip for SPR
  * Protein of interest to be immobilized. Ideally start with 10-100 ug/mL protein (need ~100 uL per surface) ...to start). Lower pH will give better immobilization, but potentially less protein function
  2 KB (302 words) - 14:43, 16 June 2010
• Protein-Lipid Overlay Assay
  [[ Category: Protein-Lipid Interactions ]]
  1 KB (184 words) - 20:55, 31 January 2011
• Preparation of Protein Lysates from Cells
  [[Category:Protein]]
  882 bytes (140 words) - 21:43, 5 January 2018

Page text matches

• Main Page
  ===Protein Analysis=== *[[Surface Plasmon Resonance - Protein Lipid Interactions]]
  4 KB (484 words) - 12:10, 15 August 2016
• Purification of GST Fusion Proteins by GSTrap
  *Express and induce protein in culture under appropriate conditions: ...ncentration of IPTG and duration of induction should be optimized for each protein)
  3 KB (440 words) - 17:55, 24 July 2012
• Bradford Assay
  *BioRad Protein Assay Dye Reagent Concentrate cat#500-0006 #Add 1-10 uL of protein sample, cover with parafilm and mix
  1 KB (202 words) - 14:39, 15 March 2018
• Surface Plasmon Resonance - Protein Lipid Interactions
  #Inject protein samples adjusting contact time as necessary to reach saturation. Typically [[Category: Protein-Lipid Interactions]]
  5 KB (753 words) - 14:40, 14 April 2011
• Western Blotting
  ##Load 3 microliters of protein ladder (purple top) (in the 4 degree), and 10 microliters of each sample in ...ert total protein stain on rocker for 5 minutes --when finished pour total protein stain back in bottle for later use!
  3 KB (410 words) - 15:58, 26 March 2021
• Purification of GST Fusion Proteins
  *Express and induce protein in culture under appropriate conditions: ...ncentration of IPTG and duration of induction should be optimized for each protein)
  2 KB (337 words) - 14:40, 12 August 2009
• Induction Conditions
  ! Protein [[Category:Protein Purification]]
  444 bytes (57 words) - 17:54, 24 July 2012
• Quantification by Absorbance at 280nm
  #Calculate extinction coefficient for protein of interest ...] tool to calculate the extinction coefficient in both molar and mg/mL for protein. Assume all Cys residues are half-cysteines.
  702 bytes (104 words) - 14:03, 11 May 2009
• Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
  Protein must be extensively dialysed in PBS to remove any amine group from the samp #Dissolve protein ligand (~1mg) in 6 mL of coupling buffer.
  2 KB (409 words) - 00:15, 27 May 2009
• Check Clones for Correct Mutation
  #Paste sequence, gi number or accession number for the refseq protein sequence into the lower box
  427 bytes (73 words) - 13:24, 27 May 2009
• Fugene Transfection of 293T/COS Cells
  #Leave mixture on Cells for 24-48h to allow protein to accumulate.
  1 KB (198 words) - 13:22, 29 August 2011
• Preparation of Protein Lysates from Mouse Tissues
  #Follow Protein Lysate instructions for Bradford Assay (see [[Bradford Assay]]) ...2X loading buffer with B-ME to each lyaste. This will generate a 2 mg/mL protein solution in SDS Loading Buffer
  1 KB (253 words) - 16:06, 25 April 2019
• MTORC1 Kinase Assay
  *Protein A/G Beads *Add 15 uL Protein A/G Beads to tubes and incubate end over end for 1h at 4C
  1 KB (179 words) - 18:15, 26 August 2009
• Using Mr Bayes to For Phlyogenetic Analysis
  #Type '''lset nst=6 rates=invgamma'''. This is for nucleotide, for protein use '''prset aamodelpr=mixed'''
  1 KB (183 words) - 14:42, 13 September 2009
• Preparation of DIG Labelled Probes
  ...n Tris, cannot have any free primary amines other than protein, we dialyze protein into PBS before labelling) *Add 350uL of PBS and 25ug of protein to an eppie.
  1,023 bytes (170 words) - 16:27, 27 October 2009
• Purification of GST-HA-S6K
  [[Category: Protein Purification]]
  1 KB (185 words) - 19:59, 20 September 2012
• Glucose Incorporation into Lipid and Glycogen
  # Do a bradford assay on 50 uL of lysed cells for protein normalization.
  3 KB (425 words) - 16:06, 30 July 2012
• Immobilizing a Protein on a CM5 Chip for SPR
  * Protein of interest to be immobilized. Ideally start with 10-100 ug/mL protein (need ~100 uL per surface) ...to start). Lower pH will give better immobilization, but potentially less protein function
  2 KB (302 words) - 14:43, 16 June 2010
• GST-EEA1 Pulldown from Yeast
  *Combine 450µL of protein with 450µL of lysates.
  940 bytes (146 words) - 17:48, 31 July 2012
• GST Pulldown Assay
  [[ Category: Protein Purification ]] [[ Category: Protein-Protein Interactions ]]
  3 KB (572 words) - 17:28, 31 July 2012

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