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→Immunoprecipitation
c. Remove and discard supernatant.
d. Wash beads 5 times with LiCl Wash Buffer, mixing 3 minutes for each wash on a rotator.
e. Add 1 ml TE Buffer to beads. Mix 1 minute on rotator and then place tubes on magnet rack to collect beads and discard supernatant.
f. Resuspend the bead pellet in 200 μl IP Elution Buffer (at room temperature). Vortex to mix.</nowiki>
3. Reverse cross-linking and recoverChIP DNA <nowiki> a. Incubate bead pellet from step 2.f above in a 65°C water bath for 1 hour, shake or vortex every 15 minutes to elute the immuno-bound chromatin from the beads. b. Spin at 14,000 rpm in a microfuge at room temperature for 3 minutes. c. Collect the supernatant, which contains the ChIP’d DNA. The tubes can be placed on the magnet to facilitate supernatant recovery. d. Incubate the supernatant containing the ChIP’d DNA in a 65°C water bath overnight to complete the reversal of the formaldehyde cross-links.</nowiki>
===Analysis of Immunoprecipitated DNA===