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TaqMan SNP Assay
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16:14, 16 November 2015
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== Protocol ==
# Dilute gDNA samples to 1-5 ng/ul.
# Place gDNA, TaqMan® Genotyping Master Mix
(40X)
, TaqMan® Genotyping Assay
(2X)
on ice.
# Make up Reaction Master mix (see table below).
TO BE COMPLETED
{| class="wikitable"
|-
! Component
! 1X volume
! 96X Volume (+ 10% excess)
|-
|TaqMan® Genotyping Master Mix (2X)
|TaqMan® Genotyping Assay (40X)
|-
|5.00 ul
|0.25 ul
|-
|528.0 ul
|26.4 ul
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Lmcallan
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