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Created page with "==Materials== *0.9 % Saline solution *10% formalin solution *Avertin *Isofluorane *30 ½ gauge needle *1 mL syringe *Peristaltic pump *Collecting dish *Surgical stage/platform..."
==Materials==
*0.9 % Saline solution
*10% formalin solution
*Avertin
*Isofluorane
*30 ½ gauge needle
*1 mL syringe
*Peristaltic pump
*Collecting dish
*Surgical stage/platform
**Make sure this is level with the edge of the collecting dish to allow for the need to rest during the procedure
===Surgical tools:===
*Dissecting scissors
*Forceps
*Clamp scissors
*Standard scissors
===Avertin Preparation===
*Taken from: http://web.jhu.edu/animalcare/rdf/avertin.html
*Stock Solution (1.6 g/ml)
**Mix 25 g avertin and 15.5 ml tert-amyl alcohol at room temperture for ~12 hours in a dark bottle.
**Can store stock solutions at room temperature for one year.
*Working solution (20 mg/ml)
**Mix 0.5 ml avertin stock solution and 39.5 ml 0.9% saline in dark/foil covered container.
**Filter solution through 0.2 micron filter into a dark/foiled covered container.
**Store solution at 4 degree C.
**Replace working solution each month.
*Notes:
**Avertin is light sensitive.
**Degredation products are lethal.
**Always store at 4 degree C
**Do NOT use a solution that is yellow or contains a precipitate.
**Avertin is lipid soluble and may require a larger dose.
*0.9 % Saline solution
*10% formalin solution
*Avertin
*Isofluorane
*30 ½ gauge needle
*1 mL syringe
*Peristaltic pump
*Collecting dish
*Surgical stage/platform
**Make sure this is level with the edge of the collecting dish to allow for the need to rest during the procedure
===Surgical tools:===
*Dissecting scissors
*Forceps
*Clamp scissors
*Standard scissors
===Avertin Preparation===
*Taken from: http://web.jhu.edu/animalcare/rdf/avertin.html
*Stock Solution (1.6 g/ml)
**Mix 25 g avertin and 15.5 ml tert-amyl alcohol at room temperture for ~12 hours in a dark bottle.
**Can store stock solutions at room temperature for one year.
*Working solution (20 mg/ml)
**Mix 0.5 ml avertin stock solution and 39.5 ml 0.9% saline in dark/foil covered container.
**Filter solution through 0.2 micron filter into a dark/foiled covered container.
**Store solution at 4 degree C.
**Replace working solution each month.
*Notes:
**Avertin is light sensitive.
**Degredation products are lethal.
**Always store at 4 degree C
**Do NOT use a solution that is yellow or contains a precipitate.
**Avertin is lipid soluble and may require a larger dose.