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Created page with "'''BDNF Primers''' ---- * oIMR0133 ---- ATG AAA GAA GTA AAC GTC CAC (Common) * oIMR0113 ---- CCA GCA GAA AGA GTA GAG GAG (WT Reverse) * oIMR01694 --- GGG AAC TTC CTG ACT AG..."
'''BDNF Primers'''
----
* oIMR0133 ---- ATG AAA GAA GTA AAC GTC CAC (Common)
* oIMR0113 ---- CCA GCA GAA AGA GTA GAG GAG (WT Reverse)
* oIMR01694 --- GGG AAC TTC CTG ACT AGG GG (Reverse)
'''BDNF Cycler Program'''
----
#94C - 10 minutes (HotStart Taq)
#94C - 30 seconds
#55C - 30 seconds
#72C - 30 seconds ----- Repeat steps 2-4 for 35 cycles
#72C - 2 minutes
#4C - Hold
----
http://jaxmice.jax.org/protocolsdb/f?p=116:2:3011685288607993::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:339,002266
----
* oIMR0133 ---- ATG AAA GAA GTA AAC GTC CAC (Common)
* oIMR0113 ---- CCA GCA GAA AGA GTA GAG GAG (WT Reverse)
* oIMR01694 --- GGG AAC TTC CTG ACT AGG GG (Reverse)
'''BDNF Cycler Program'''
----
#94C - 10 minutes (HotStart Taq)
#94C - 30 seconds
#55C - 30 seconds
#72C - 30 seconds ----- Repeat steps 2-4 for 35 cycles
#72C - 2 minutes
#4C - Hold
----
http://jaxmice.jax.org/protocolsdb/f?p=116:2:3011685288607993::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:339,002266