Changes

Splitting Cells

16 bytes added, 15:30, 6 May 2009

no edit summary

==Materials==

*~~FBS~~ Media (L1-FBS for 3T3-L1, COS-FBS for others): Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM

*PBS -/-

*0.05% Trypsin

==Protocol==

#Warm PBS and Mediain water bath

#Wash cells twice with 10 mL (per 10 cm dish) PBS -/-

#Add 1 mL trypsin and sit in the hood

edits