*Plasmid amplification use subcloning efficiency DH5a*Cloning use OneShot TOP10 (Pink)*Mutagenesis use XL1 Blue Supercompetent (Blue), comes in 50uL aliquots*SOC Bufferor LB Media
*DNA of interest (1 uL for plasmid, 5 uL for cloning/mutagenesis)
*Plates (Amp or Kan; in cold room, see [[ Making LB Agar Plates ]])