Changes

Triglyceride Assay from Cells and Tissues

No change in size, 17:10, 28 June 2013
m
Protocol: Volume of butanol mixture
# Dry in fume hood overnight (or until completely dry)
# If absorbance is going to be measured by cuvette, use non-bolded values. If you are using a plate reader use bolded values.
# Add 50uL 500uL '''(500uL50uL)''' of '''Butanol Mixture'''. See [[#Suggested Volumes | Suggested Volumes]] for your specific tissue
# Measure triglyceride levels using Sigma Diagnostic Kit using 5 uL of sample:
## Resuspend triglyceride and glycerol reagent with water if necessary.
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