915
edits
Changes
updated vehicle information
==Materials==
*Tween 80 - Sigma P1754
*PEG 400 Liquid - Sigma P3265
==Preparation==
*Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. This is 5.2% PEG/Tween
*Dissolve rapamycin at 20 mg/mL in water.
*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).