Changes

GST-EEA1 Pulldown from Yeast

20 bytes added, 17:48, 31 July 2012

added link to 2xHNG

== '''Materials''' ==

*[[ 2xHNG_Buffer | 2x HNG ]] (100mL). Combine 100mM HEPES (10mL), 300mM NaCl (7.5mL), 20% glycerol (20mL)

*1x HNG (10mL). Mix 5mL 2xHNG and 1 PI tablet.

*Lysis Buffer (10mL). Combine 5mL 1x HNG, 1 PI tablet, 1mL of 1% NP40, and 50µL of magnesium chloride.

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