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#*Run PCR Program. Normally use touchdown PCR ('''DAVETD''') as follows:
#*Purify PCR product if necessary using Qiagen kit (Add 5x PB)
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==Protocol==
#Use the following volumes per reaction
::*Buffer, 5 uL of 10X buffer ::*Primers, 10uL of 1uM stock solution in water (both primers combined)::*dNTPs, 5uL of 2 mM ::*Sterile water, 28 uL::*Template 1 uL*::Polymerase 1 uL
##1 min at 94
##30s at 65
##11 min at 72
##hold at 4 until ready