915
edits
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[[Category:Mice]]
[[Category:Animal Studies]]
[[Category:Drug Treatment]]
[[Category:Insulin Sensitivity]]
[[Category:Insulin Signaling]]
[[Category:Metabolism]]
We use water-soluble Dex from Sigma (D2915). It says on the bottle how many mg/g is actually Dex. The calculations below are for a bottle where the powder was 65mg/g. Dex
==For i.p. injection 5 mg/kg==
* Makes 15mL stock solution of 1mg/mL
* dissolve 0.231g Dex powder in 15mL PBS
<pre>BW (g) X 0.005 = injection volume (mL)</pre>
===Calculation===
<pre>
65mg dex/ = 15mg dex/ = 0.231g
1000mg powder ? mg powder
</pre>
==For drinking water administration ~1mg/kg/day==
* Makes 1L of drinking water
* dissolve 0.092g in 1L water
===Calculation===
* 1mg Dex/kg/day because mice drink ~5mL /day so want 0.2 mg Dex/kg/mL
* average mouse weighs 0.03kg so want 0.006mg Dex/mL or 6mg/L
<pre>
65mg dex/ = 6mg dex/ = 0.092g
1000mg powder ? mg powder
</pre>
*This assumes that the mice are about 30g and they drink 5mL/day.
[[Category:Animal Studies]]
[[Category:Drug Treatment]]
[[Category:Insulin Sensitivity]]
[[Category:Insulin Signaling]]
[[Category:Metabolism]]
We use water-soluble Dex from Sigma (D2915). It says on the bottle how many mg/g is actually Dex. The calculations below are for a bottle where the powder was 65mg/g. Dex
==For i.p. injection 5 mg/kg==
* Makes 15mL stock solution of 1mg/mL
* dissolve 0.231g Dex powder in 15mL PBS
<pre>BW (g) X 0.005 = injection volume (mL)</pre>
===Calculation===
<pre>
65mg dex/ = 15mg dex/ = 0.231g
1000mg powder ? mg powder
</pre>
==For drinking water administration ~1mg/kg/day==
* Makes 1L of drinking water
* dissolve 0.092g in 1L water
===Calculation===
* 1mg Dex/kg/day because mice drink ~5mL /day so want 0.2 mg Dex/kg/mL
* average mouse weighs 0.03kg so want 0.006mg Dex/mL or 6mg/L
<pre>
65mg dex/ = 6mg dex/ = 0.092g
1000mg powder ? mg powder
</pre>
*This assumes that the mice are about 30g and they drink 5mL/day.