Changes

*0.07U/mL humulin in PBS (make as 70uL of 100 U/mL in 10 mL, sterile filtered). This will correspond to 0.7mU/kg injections.

Timer

*Remove food from mice for about 6h. Add do not feed tag to cages, or move cage to procedure room.

*Bring mice to procedure room. Put water bottle on cage.