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*Revive Yeast Cells (2-3) days
*Pick one colony and make a 1/2 inch x 1/2 inch square patch of yeast on selective plates, allow to grow for 1 day. (24°C)
*Place nitrocellulose filters on gel and incubate overnight at 23°C. (Make sure to roll the sheet with a tube)
*Wash filter in water.
*Block filter with BSA for 1 hour.
*Wash in primary antibody overnight at 4C or >3h at room temp
(10,000x dilution)
*Rinse in Tbst.
*Secondary antibody at 10 000X for 45min-1h.
*Rinse in TBST.
*Develop