Open main menu

Changes

PCR Amplification of DNA (KOD Polymerase)

75 bytes removed, 21:24, 3 November 2009
no edit summary
*dNTPs – dilute to 2 mM each in water, make single use aliquots (50 uL aliquots... 10uL of each dNTP, 460uL water)
*Template – generally 1uL or less of a plasmid miniprep
*KOD Polymerase – use Pfu Turbo for cloning and Taq for noncloning. Use appropriate buffer.
==Protocol==