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*Protein A/G Beads
*[[CHAPS Lysis Buffer]]
*[[mTORC1 TORC1 Kinase Buffer]]
==Protocol==
*Add 15 uL Protein A/G Beads to tubes and incubate end over end for 1h at 4C
*Spin down beads for 15s at 14 000 RPM and aspirate with a fine-tipped (or crushed tip) pipet tip
*Wash beads 2x with Lysis Buffer and 1x with [[TORC1 Kinase Buffer ]] (-ATP)
*Add ATP (250 uM) and GST-S6K (200ng/rxn) to kinase buffer , 20 uL per reaction.
*Incubate at 30C for 30 min.