Changes

Finding and Ordering BAC Clones

347 bytes added, 15:26, 27 July 2009

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#Zoom out as necessary to find ends of BAC clones

#Print out and save BAC clone choices

==Find Clone with BLAST==

#Go to blast website (http://www.ncbi.nlm.nih.gov/blast)

#Select Mouse (or whichever species) under BLAST assembled genomes

#Select clone end sequences under database

#Select appropriate program (BLASTN if using a nucleotide query, TBLASTN if using a protein query)

#Enter sequence or accession number in the search box

==Ordering BAC Clones==

#Go to BACPAC website [(http://bacpac.chori.org/order_clones.php])

#Enter clone name and submit to verify

#Can order clones through LSI purchasing (vendor is listed as BACPAC-chor)

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