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Buffer/RIPA

59 bytes added, 13:33, 8 July 2009
mistake with NaF and NaPPi concentrations. Added note about NaVO3 location
==RIPA Buffer (for 10mL lysis buffer)==
{| border="1"
! || Final Concentration || per 10 mL || Stock|| Location
|-
|Tris pH7.4 || 50mM || 500uL || 1M
|EDTA || 1mM || 20uL || 0.5M
|-
|NaVO3 || 1mM || 100uL || 1M|| Biochemical Stocks (-20)
|-
|NaF || 5mM || 100uL || 250mM500mM
|-
|NaPPi || 25 mM || 1 mL || 500mM250mM
|-
|- Protease Inhibitors !! || || 1 tabmini tablet || || 4C
|}