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PCR Analysis of Tail DNA

335 bytes added, 16:27, 5 June 2009

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==Protocol==

Use the following volumes per reaction

Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer)

Forward Primer: .4ul

Reverse Primer: .4ul

dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer)

Sterile water: 13.6 uL

Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer)

Template: 1 uL

see [[Preparing an Agarose Gel]] for details on preparing a DNA gel

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