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#Remove stainless steel bead or transfer the homogenized tissue to a new tube.
#Centrifuge at 14 000 RPM at 4C for 10 min
#Remove supernatant to and place into clean tube. If lysing fat, try to avoid the floating fat cake. If necessary respin to clarify
#Follow Protein Lysate instructions for Bradford Assay (see [[Bradford Assay]])
#Prepare samples for gels by adding 800 ug protein to a final volume of 200 uL of lysis buffer. Add 200 uL of 2X loading buffer with B-ME to each lyaste. This will generate a 2 mg/mL protein solution in SDS Loading Buffer