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→Prior to starting this section:
===== Prior to starting this section: =====
* Bring 1 M NaHCO3 to room temperature. A precipitate may be observed but will go into solution once room temperature is achieved. The 1 M NaHCO3 can be vortexed.
* Set water bath to 65°C for use in Section Elater.29. Make Elution Buffer for all IP tubes as well as all Input tubes (see Section C, step 7).
* For each tube, prepare 200 μL of elution buffer as follows: 10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water.
* OR can make this way [[ChIP Elution Buffer]]
* Alternatively, make a large volume to accommodate all tubes. For example, if there are 10 tubes mix together 105 μL 20% SDS, 210 μL 1M NaHCO3 and 1.785 mL sterile, distilled water.
30. For Input tubes (see Section C, step 7), add 200 μL of Elution Buffer and set aside at room temperature.
31. Add 100 μL of Elution Buffer to each tube containing the antibody/agarose complex. Mix by flicking tube gently.