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Chromatin Immunoprecipitation

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supernatant fraction.
# Wash the Protein G Agarose-antibody/chromatin complex by resuspending the beads in 1 mL each of the cold buffers in the order listed below and incubating for 3-5 minutes on a rotating platform followed by brief centrifugation (3000-5000 x g for 1 minute) and careful removal of the supernatant fraction:
a. * Low Salt Immune Complex Wash Buffer (Catalog # 20-154), one washb. * High Salt Immune Complex Wash Buffer (Catalog # 20-155), one washc. * LiCl Immune Complex Wash Buffer (Catalog # 20-156), one washd. * TE Buffer (Catalog # 20-157), two washes
D. Elution of Protein/DNA Complexes
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