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Buffer/RIPA

424 bytes added, 00:29, 27 May 2009
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==RIPA Buffer (for 10mL lysis buffer)==
{| border="1"
! || Final Concentration || per 10 mL || Stock
|-
|Tris pH7.4 || 50mM || 500uL || 1M
|-
|Na Deoxycholate || 0.25% || 250uL || 10%
|-
| NP-40 || 1% || 1mL || 10%
|-
|NaCl || 150mM || 375uL || 4M
|-
|EDTA || 1mM || 20uL || 0.5M
|-
|NaVO3 || 1mM || 100uL || 1M
|-
|NaF || 5mM || 100uL || 250mM
|-
|NaPPi || 25 mM || 1 mL || 500mM
|-
|- Protease Inhibitors !! 1 tab
|}