Rab5 FRET Assay
Materials
- mCitrine-Rab5BD (from EEA1/NT)
- mCFP-Rab5A
- Acid etched Coverslips (see Acid Etching Coverslips)
- Cells of interest
Protocol
- Plate out ~150 000 cells per well in a 6 well dish with an acid etched coverslip
- The next morning transfect cells with Fugene, Lipofectamine or other protocol
- That evening replace with fresh media (and serum starve if doing insulin/growth factor stimulation