Dilution Formula

C1V1=C2V2

• C1=measured absorption
• V1=unknown volume to be added to final volume
• V2=desired volume to re-suspend V1 in (usually 4mL)
• C2=OD/2^x

• x=amount of times cells will double
• find this by dividing the number of hours the cells will be growing by how long one doubling period is