Primer Design for qPCR

Finding Known Primer Sets

Find your RNA sequence on entrez by going to http://www.ncbi.nlm.nih.gov/nuccore (make sure its the correct species)
Under analyse this sequence click Pick Primers
Under PCR Product Size pick 70-150 as the range
Under Exon/intron selection -> Intron Inclusion check the box by Primer pair must be separated by at least one intron on the corresponding genomic DNA
If you want to look at a specific region of the mRNA and an appropriate primer pair in that region is not selected, change the range under PCR template on the top
Click Get Primers on the bottom and wait for results
Print this out, for future reference and when you order primers name them as follows species-Gene-seq.start-seq.end, for example mm-SREBF1-127-254.
Enter both primers in ExperimentDB