Rab5 FRET Assay

Revision as of 14:18, 16 September 2010 by Davebridges (Talk | contribs) (wrote initial protocol)

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Revision as of 14:18, 16 September 2010 by Davebridges (Talk | contribs) (wrote initial protocol)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Materials

mCitrine-Rab5BD (from EEA1/NT)
mCFP-Rab5A
Acid etched Coverslips (see Acid Etching Coverslips)
Cells of interest

Protocol

Plate out ~150 000 cells per well in a 6 well dish with an acid etched coverslip
The next morning transfect cells with Fugene, Lipofectamine or other protocol
That evening replace with fresh media (and serum starve if doing insulin/growth factor stimulation

Last modified on 16 September 2010, at 14:18