FDB Muscle Dissection from Mice

Revision as of 19:08, 13 June 2017 by Snyderds (Talk | contribs)

Revision as of 19:08, 13 June 2017 by Snyderds (Talk | contribs)

This protocol is modified from section 3.1.2 of Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells and the first part of the following video with accompanying protocol DNA Electroporation, Isolation and Imaging of Myofibers | Protocol.

Materials

  • Tweezers
  • Standard operating scissors
  • Surgical scissors
  • Forceps
  • Dissection stage with 7 pins
  • Anesthetic

Flexor Digitorum Brevis Muscle Extraction

  • Sacrifice the animal with anesthetic gas inhalation followed by cervical dislocation to ensure death.