FDB Muscle Dissection from Mice

This protocol is modified from section 3.1.2 of Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells and the first part of the following video with accompanying protocol DNA Electroporation, Isolation and Imaging of Myofibers | Protocol.

Materials

• Tweezers
• Standard operating scissors
• Surgical scissors
• Forceps
• Dissection stage with 7 pins
• Anesthetic

Flexor Digitorum Brevis Muscle Extraction

• Sacrifice the animal with anesthetic gas inhalation followed by cervical dislocation to ensure death.