User contributions
10 June 2016
Chromatin Immunoprecipitation for Tissue Samples
no edit summary
+1,211
Chromatin Immunoprecipitation for Tissue Samples
Created page with "The tissue fixation portion of this protocol was taken from the Ren Lab ENCODE protocol for brown adipose tissue and can be found here [https://www.encodeproject.org/document..."
Chromatin Immunoprecipitation
no edit summary
+36
17 May 2016
Designing Clones Using Benchling
no edit summary
-1
Designing Clones Using Benchling
Created page with "First, go to [https://benchling.com] to create an account. The following protocol is a tutorial for designing clones using the Gibson method: #Click the "Create+" tab at the t..."
23 March 2016
Chromatin Immunoprecipitation
Incubate bead-antibody complex with fragmented, cross-linked chromatin
+1
Chromatin Immunoprecipitation
Incubate bead-antibody complex with fragmented, cross-linked chromatin
+46
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+16
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+255
Chromatin Immunoprecipitation
Purification of ChIP DNA
+24
21 March 2016
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
-20
Chromatin Immunoprecipitation
Incubate bead-antibody complex with fragmented, cross-linked chromatin
-11
Chromatin Immunoprecipitation
Reverse cross-linking and recover ChIP DNA
-15
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+3
Chromatin Immunoprecipitation
Buffers and Solutions Needed
+32
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+6
Chromatin Immunoprecipitation
Incubate bead-antibody complex with fragmented, cross-linked chromatin
-4
Chromatin Immunoprecipitation
Reverse cross-linking and recover ChIP DNA
+6
Chromatin Immunoprecipitation
Analysis of Immunoprecipitated DNA
-25
Chromatin Immunoprecipitation
Reverse cross-linking and recover ChIP DNA
+1,117
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+184
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
+76
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
+22
Chromatin Immunoprecipitation
Buffers and Solutions Needed
+22
Chromatin Immunoprecipitation
Incubate bead-antibody complex with fragmented, cross-linked chromatin
+12
Chromatin Immunoprecipitation
Buffers and Solutions Needed
-1
Chromatin Immunoprecipitation
Buffers and Solutions Needed
+27
1 February 2016
28 January 2016
Preparation of RNA Samples from Mouse Tissues
Protocol
-1
Preparation of RNA Samples from Mouse Tissues
Protocol
+40
26 January 2016
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
Chromatin Immunoprecipitation
Incubate bead-antibody complex with fragmented, cross-linked chromatin
+5
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
+294
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
-1
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
+73
25 January 2016
RT-PCR primer design for ChIP
no edit summary
-23
RT-PCR primer design for ChIP
no edit summary
RT-PCR primer design for ChIP
no edit summary
+6
Locating ChIP-seq peaks from ENCODE
Created page with "# Go to the UCSC website [https://genome.ucsc.edu/index.html] # Go to the ENCODE option on the left-hand side of the page # Click on the Explore ENCODE data at UCSC picture #..."
Chromatin Immunoprecipitation
Analysis of Immunoprecipitated DNA
+38
RT-PCR primer design for ChIP
Created page with "# Locate the potential gene regions you believe your protein is bound to (for glucocorticoid-induced ChIP-seq peaks refer to Locating ChIP-seq Peaks protocol). Make sure this..."
20 January 2016
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
-1
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
-12
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
+15
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
+1
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
+4
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
+69
Chromatin Immunoprecipitation
Immunoprecipitation
+581
Chromatin Immunoprecipitation
Immunoprecipitation
+1,683