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• Main Page
  *[[PCR Amplification of DNA]] *[[Cesium Chloride Preparation of DNA]]
  4 KB (484 words) - 12:10, 15 August 2016
• Restriction Enzyme Based Cloning
  :*Add to pink purification column, wash and elute in 30 uL #Add 1 uL T4 DNA Ligase (Invitrogen).
  1 KB (227 words) - 14:40, 7 May 2009
• Purification of GST-HA-S6K
  *GST-HA-S6K1 plasmid. Prepare by [[Cesium Chloride Preparation of DNA]]. Need 500 ug per 10 plates of cells #Combine 500 ug DNA with 10 mL OptiMEM, and separately 700 uL Lipofectamine 2000 with 10 mL Opt
  1 KB (185 words) - 19:59, 20 September 2012
• Generating and Amplifying Adenoviral Constructs
  ===Linearization and Purification of Shuttle DNA=== #Digest 0.2-0.5 ug plasmid DNA in 100 uL volume with 30-100U of PmeI overnight at 37C. If desired check a
  6 KB (872 words) - 20:56, 11 July 2012
• Vinexin Genotyping
  [[ Category:DNA]] ...e targeted allele only. For Southern blotting, SalI/EcoRI-digested genomic DNA was probed with a 0.4-kb fragment immediately upstream of the 5′ arm (Sup
  2 KB (225 words) - 15:14, 6 August 2012
• Extraction of DNA from TRIZOL preparations
  This protocol is for extracting DNA from TRIZOL preparations after the RNA-containing upper phase has been comp ...sion or use the shaker. '''NEVER''' vortex samples, as it will destroy the DNA.
  3 KB (417 words) - 17:42, 25 September 2015
• Chromatin Immunoprecipitation
  ...DS, Mortazavi A, Myers RM, Wold B. Genome-Wide Mapping of in Vivo Protein-DNA Interactions. Science (80- ) 316: 1497–1502, 2007. [http://dx.doi.org/10. * QIAquick PCR Purification Kit
  11 KB (1,742 words) - 15:23, 10 May 2018
• Chromatin Immunoprecipitation for Tissue Samples
  ==== Reverse cross-linking and recover ChIP DNA ==== # Collect the supernatant, which contains the ChIP’d DNA. The tubes can be placed on the magnet to facilitate supernatant recovery.
  6 KB (1,077 words) - 21:43, 10 June 2016